YUYU Active的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到附近那裡買和營業時間的推薦產品

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磁性奈米攜藥載體的生物分佈及血液動力學效應

為了解決YUYU Active的問題，作者邱千彧 這樣論述：

Recommendation Letter from the Thesis Adivisor……………………….Thesis Oral Defense Committee Certification……………………………Acknowledgement…………………………………………………….…iii摘要………………………………………………………..….................ivAbstract…………………………………..……………….....…………...vContent……………………………………………...…….…...................viList of Fi

gures…………………………………………………...…..…...ixList of Tables……………………………………………………….…….xAbbreviation Table..……………………………………...………….…..xiChapter 1 Background………………………………………...….……11.1 Application of nanoparticles…………………...………..............11.2 Application of magnetic nanoparticles (MNPs) as drug carriers………………………

…………………………………..…...11.3 Interaction of MNPs and plasma proteins…………………..…...31.4 Composition of drug-loaded MNPs…………………………..…51.5 Magnetic guiding strategy in vivo…………………………...…..81.6 Interactions between endothelial cells and MNPs…………..…...91.7 Bio-distribution and toxicity of MNPs…………………….…...10Ch

apter 2 Specific Aims ……………………………………………....13Chapter 3 Materials and Methods ………………………………..….143.1 Materials……………………………………………………..…143.2 Cremaster muscle preparation……………………….…………143.3 Tissue flow measurement.………………………………….…..163.4 Laser Speckle Imaging…………….……………………………173.5 Capillaroscopy…………………………

………………….…....173.6 In vitro vessel simulation……………………………..……...…183.7 In vitro circulation system with rat blood…………...…..…...…193.8 Production of particle-free serum………………………………203.9 In vitro circulation system with serum………………………….203.10 Endothelial cell culture…..……………..…………....………..213.11 Applic

ation of magnetic force……………………..……..…...213.12 KSCN assay for iron content analysis…………………….......213.13 Size and ζ-potential measurement of nanoparticles…………...223.14 Histology…………………………………………….……..….223.15 Statistical analysis…………………………………..……....…25Chapter 4 Results………………………………………………..……..264.1

Hemodynamic effect of magnetic nanoparticle with different coating material…………………………………………….....…….264.1.1 Magnetic guiding induced particle retention…........................264.1.2 Magnetic capture of PEG (-) MNP in microvessels……….....274.1.3 Blood flow measurement of MNPs with or without PEG…..

..284.1.4 Dynamic retention of PEGylated MNPs……………….…..…294.1.5 PEGylation facilitated MNP escape in vitro ………………....304.1.6 Magnetic capture of MNPs in circulating flow in vitro………324.1.7 Magnetic capture of MNPs in circulating serum in vitro……..324.1.8 PEGylation attenuate MNP interaction with end

othelial cells..334.1.9 Characterization of MNPs………………………………….…344.2 Biodistribution of MNP after i.v. administration of MNP-rtPA..344.2.1 Hepatic retention of MNP-rtPA………………………………354.2.2 Tissue retention of MNP-rtPA in the lung and spleen……...…364.2.3 Immunohistochemistry staining of macrophage…………

…...36Chapter 5 Discussion…………………………………………………..38Chapter 6 Conclusion………………………………………………….44Chapter 7 Future Work…………………………………………….….45References……………………………………………………………….46Tables……………………………………………………………………64Figures………………………………………………………….………..66List of FiguresFigure 1. The illustration of rat cremaster

muscles……………..………66Figure 2. Magnetic guiding induced particle retention in microvessels..67Figure 3. Representative capture of MNP in a microvessel with time….68Figure 4. Magnetic capture of MNP and subsequent flow changes…….69Figure 5. Hemodynamic effects of magnetic capture of PEGylated MNPs…………

………………………………………………………....70Figure 6. Dynamic retention of PEGylated MNP in vessels…………....72Figure 7. PEGylation facilitated MNP escape in vitro system………….73Figure 8. Circulated flow interaction with PEGylated or non-PEGylated MNP under magnetic field…………………………..………………......74Figure 9. Circulated s

erum interaction with PEGylated or non-PEGylated MNP under magnetic field………………..………………………….….75Figure 10. PEGylation attenuated MNP interaction with endothelial cells………………………………………………………………….…..76Figure 11. Characterization of MNPs……………………………...……77Figure 12. Hepatic retention of MNP-rtPA………………………

……..78Figure 13. Tissue retention of MNP-rtPA in the lung and spleen…..…..79Figure 14. Immunohistochemistry staining of macrophage………..…...80Figure 15. Summary diagrams of interactions of blood components and MNPs with (+) or without (-) PEG under the influence of magnetic and hemodynamic forces in

perpendicular direction in vivo ………………..81List of TablesTable 1. Characteristics of magnetic nanoparticles in H2O…………….64Table 2. Correlated shear rate and shear stress as a function of flow.….65